Nextseq 550 user guide DRAGEN for Illumina DNA Prep with Enrichment Dx on NextSeq 550Dx Application User Guide Welcome to the Nextseq 500/550 & Dx instructional video series. The integration allows for automated tracking of an Illumina sequencing run in Clarity LIMS, which includes tracking instrument run status, generating run A NextSeq 500/550 Kit cannot be used for a diagnostic mode run. iScan. We have 3 illumina NextSeq 550Dx manuals available for free PDF download: Instrument Reference Manual, Instrument Safety And Compliance Manual . Data concordance between the NextSeq 1000/2000 and NextSeq 550 Sequencing Systems Download: Application note < 1 MB: Mar 23, 2020: Illumina Sequencing Platforms NextSeq® 550 Sequencing System Datasheet Author: Illumina Subject: Fast, flexible, high-throughput desktop sequencer enables transcriptome and targeted resequencing applications with the accuracy of Illumina SBS technology. Cluster Optimization Overview Guide. Lab specifications and requirements to prepare a site for the NextSeq 550Dx System. 1 supports the integration of BaseSpace Clarity LIMS to Illumina NextSeq 500 and 550 sequencing systems. 2 billion a: Maximum read length: 2 x The NextSeq 550 System features an intuitive user interface and load-and-go design that allow users to perform various sequencing applications with minimal training and instrument set-up time. Support Center / DRAGEN for Illumina DNA Prep with Enrichment Dx on NextSeq 550Dx Application User Guide. For specific trademark information, NextSeq 1000/2000 Product Documentation. 2, 2022-11-01. 0 Local Run Manager v4. Page 5 of 26 II. Manual denaturation of libraries on the NextSeq 1000/2000. How to NextSeq 500/550 Integration v2. The NextSeq 550Dx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) The NextSeq 550 System features an intuitive user interface and load-and-go design that allow users to perform various sequencing applications with minimal training and instrument set-up time. Support Resources. This video will guide you through the necessary steps to take when wanting to perform a Maintenance or Manual Post-Run wash on the Nextseq 500/550 & Dx. Instructions for sequencing with custom primers on the NextSeq 500 and NextSeq 550 Systems. Additionally, the seamless integration with NextSeq 550Dx on NextSeq 550Dx Application u ser Guide Array scanning in Research Mode As with the NextSeq 550 System, the NextSeq 550Dx NextSeq 550Dx Instruments enables user-friendly sequencing run setup and secondary data analysis. All trademarks are the property of Illumina, Inc. Updated user-supplied consumables to specify uses of NaOCl for View online (73 pages) or download PDF (1 MB) illumina NextSeq 550Dx User manual • NextSeq 550Dx PDF manual download and more illumina online manuals Chat with manual Explore directory For more information, see the NextSeq 500/550 Kit v2 Reference Guide (document # 15058065). . RFID Reader - Model # TR-001-44 User Guide (document # 15041950) Provides information about the RFID reader in the instrument, compliance certifications, and safety considerations. The NextSeq 550 System offers: • Ready-to-use reagents Water,laboratory-grade Generallabsupplier Washingtheinstrument(manual wash) Reagentor spectrophotometric-grade methanolorisopropyl alcohol(99%),100ml bottle Generallabsupplier Cleaningopticscomponents periodicallyandsupportthe objectivecleaningcartridge Airfilter Illumina,catalog# 20063988 Forinstrumentswithanairfilter accessiblefromtherearpanel. Note that this integration also includes a preconfigured Library Prep Validation v1. Instructions for operating and maintaining the NextSeq 500 and NextSeq 550 instruments. Products Learn Company Support Recommended Links. Ready-to-use panels save users the time and effort of identifying targets, designing primers, and optimizing panels. 5, prepared in previous step • 1x NextSeq™ 500/550 Buffer Cartridge v2 (75 cycles), at room temperature 2. Embgenix™ PGT-A Kit (RUO) User Manual for Illumina® NextSeq® 500/550 System (080122) takarabio. Reference Material Guidelines for managing the security of the control computer on your sequencing system. The NextSeq 550Dx Instrument is not intended for whole genome or de novo sequencing. 12% Tween 20 Sigma-Aldrich, catalog # P7949 Washing Guidelines for managing the security of the control computer on your sequencing system. 5. Created Date: 3/3/2020 3:02:03 PM How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. How to set up a PhiX validation run on the NextSeq 500/550 in manual mode. Instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the NextSeq 500 and 550 Systems. • 1x NextSeq™ 500/550 High Output Flow Cell v2. nextseq-1000-2000 Systems configured for manual mode with Run Monitoring and Storage in BaseSpace Sequencing Hub require a sample sheet. 0 Real-Time Analysis v2. Troubleshooting NVRAM or Socket Errors on NextSeq 500/550. NextSeq 550 System Guide (document # 15069765) Provides instructions for operating the instrument and troubleshooting procedures. It also supports importing and exporting sample sheets. MiniSeq System HiSeq. High-throughput sequencing— The NextSeqTM 550Dx instrument View and Download illumina NextSeq 550Dx instrument reference manual online. Page 19 NextSeq 550Dx Research Mode Instrument Reference Guide User-Supplied Consumables for Instrument Maintenance Consumable Supplier Purpose NaOCl, 5% Sigma-Aldrich, catalog # 239305 Washing the instrument using the How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. Flushes the system with a user-supplied wash solution of laboratory-grade water and Tween 20 (buffer wash cartridge). Troubleshooting Cycle 1 Error, Camera Disabled, Failed to Detect Clusters on the NextSeq 500/550. Not for use in diagnostic procedures. Sequencing. For more information, see the NextSeq 500/550 Kit v2 Reference Guide (document # 15058065). The NextSeq 550 System offers: • Ready-to-use reagents RESULT IN DAMAGE TO THE PRODUCT(S), INJURY TO PERSONS, INCLUDING TO USERS OR OTHERS, AND DAMAGE TO OTHER PROPERTY, AND WILL VOID ANY WARRANTY APPLICABLE TO THE PRODUCT(S). NextSeq 550: Run Quality and Best Practices All trademarks are the property of Illumina, Inc. The workflow contains a single-step protocol that models the library prep required to produce normalized libraries that are ready for the NextSeq 500/550 Sequencing v1. 7. This video will guide you through the necessary steps to take when setting up a run on the Ne The NextSeq 550 System features an intuitive user interface and load-and-go design that allow users to perform various sequencing applications with minimal training and instrument set-up time. 3 in the v2 reagent cartridge. Recommended panels for the NextSeq 550 System The NextSeq 550 amplicon sequencing solution supports targeted resequencing of fixed or custom gene panels. • Required every 14 days that the instrument is idle with reagent cartridge and buffer cartridge in place. • NextSeq 500 and NextSeq 550 Sequencing Systems. Thawing and reagent preparation guidelines for the NextSeq 1000/2000 reagents. 1 Set up sequencing run parameters During this step, sequencing run parameters are selected on the NextSeq™ 550. Jul 13, 2023. 1. Perform a Manual Wash. NextSeq 1000 and NextSeq 2000 Reagents. 9. Instructions for denaturing and diluting prepared libraries for sequencing. b. Minimum input for high-quality RNA shown; 10 ng minimum recommended for optimal quality and FFPE NextSeq 550 User Guide (document # 15069765) Provides an overview of the NextSeq 550 next generation sequencing instrument components, instructions for operating the instrument, and maintenance and troubleshooting procedures. This video will guide you through the necessary steps to take when setting up a run on the Nextseq 500/550 & Dx. Cloud Software User-Supplied Consumables. Page 11 NextSeq 550Dx Instrument Reference Guide User-Supplied Consumables for Instrument Maintenance Consumable Supplier Purpose NaOCl, 5% Sigma-Aldrich, catalog # 239305 Washing the instrument using the (sodium hypochlorite) (or laboratory-grade equivalent) manual post-run wash; diluted to 0. HiSeq 1000 System All trademarks are the property of Illumina, Inc. NextSeq 500/550 On Premises Software. Protective Earth. Products. The NextSeq 550 System offers: • Ready-to-use reagents For more information, refer to the Sequencing Analysis Viewer User Guide All trademarks are the property of Illumina, Inc. Tecan. For specific trademark information, How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. Updated user-supplied consumables to specify uses of NaOCl for manual wash options introduced in in the v2 reagent cartridge. Revision History. NextSeq 1000 System NextSeq 2000 System NextSeq 500 System NextSeq 550 System NextSeq 550Dx Instrument MiniSeq. 2 Document note The Olink® Explore User manual, doc nr 1153, is obsolete, and has been replaced by the following documents: Olink ® Explore Overview User Manual, doc nr 1187 Olink ® Explore 384 User Manual, doc nr 1188 How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. Getting Started. Type & Size. On the NextSeq™ 550 Home screen, select Experiment. NextSeq 550 System, this solution can help more labs add Users can choose a. NextSeq 500 and NextSeq 550 Sequencing Systems. Denature and Dilute Protocol Generator. Overview. Overview; Documentation; Training; More. How to denature and dilute libraries on the NextSeq 500/550. Hands-on and total time based on manual processing of up to 24 samples for Illumina Stranded Total RNA and mRNA workflows and 1 sample on enrichment workflow. in the v2 reagent cartridge. The instrument has a connection to protective earth through the enclosure. Water,laboratory-grade Generallabsupplier Washingtheinstrument(manual wash) Reagentor spectrophotometric-grade methanolorisopropyl alcohol(99%),100ml bottle Generallabsupplier Cleaningopticscomponents periodicallyandsupportthe objectivecleaningcartridge Airfilter Illumina,catalog# 20063988 Forinstrumentswithanairfilter accessiblefromtherearpanel. Instructions for using the NextSeq 550Dx instrument. How to create Local Run Manager credentials to perform a NextSeq 500/550 system check in NCS 4. The Illumina NextSeq 500 and NextSeq 550 systems combine the power of high-throughput sequencing with the simplicity of a The instructions in this guide are also applicable to the NextSeq 550Dx instrument when in research mode with the latest version of RUO instrument software. Minimum input for high-quality RNA shown; 10 ng minimum recommended for optimal quality and FFPE NextSeq 500 and NextSeq 550 Sequencing System Guide System overview and instructions for operating and maintaining the NextSeq 500 and NextSeq 550. NextSeq 550Dx laboratory equipment pdf manual download. Microlab STAR Line Operator’s Manual, Hamilton Doc ID 624668. 0 workflow. This instrument reference guide provides instructions on the use of the NextSeq 550Dx instrument in research (RUO) mode. Scanning (NextSeq 550 only) All trademarks are the property of Document Date DescriptionofChange Document# 1000000009513 v03 March2019 Addedinformationaboutv2. 1 User and Configuration Guide. Technical Support. MiSeq. 0 workflow, used for validation purposes only. Updated user-supplied consumables to specify uses of NaOCl for NextSeq 500 and NextSeq 550 Sequencing Systems Custom Primers Guide (15057456) Author: Illumina Subject: Instructions for using custom primers on the NextSeq 500 and NextSeq 550 systems. How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. 0. 11. NextSeq 550 System. How to set up a run on the NextSeq 500/550 Video. NextSeq 500 and NextSeq 550. Cloud Software Information about preparing the lab for the NextSeq 500 and NextSeq 550 systems. All of the hazards described herein can be avoided by following the standard operating procedures included in the NextSeq 500 System Guide (part # 15046563) or NextSeq 550 System Guide (part # 15069765). 2. Viewing NextSeq 1000/2000 runs in Sequencing Analysis Viewer. using NextSeq™ 550 User Manual 1192, v1. Use these guidelines to configure your system and ensure a more secure operating environment. Date. Additionally, the seamless integration with NextSeq 550Dx on NextSeq 550Dx Application u ser Guide Array scanning in Research Mode Manual denaturation of libraries on the NextSeq 1000/2000. or their User-SuppliedConsumablesforInstrumentMaintenance Consumable Supplier Purpose NaOCl,5% (sodiumhypochlorite) Sigma-Aldrich,catalog# 239305 (orlaboratory-grade As with the NextSeq 550 System, the NextSeq 550Dx NextSeq 550Dx Instruments enables user-friendly sequencing run setup and secondary data analysis. Local Run Manager enables a streamlined, user-friendly interface for setting up samples, runs, and analysis, and for viewing analysis results. illumina NextSeq 550Dx Instrument Reference Manual (75 pages) Research Mode. X. Information about preparing the lab for the NextSeq 550Dx instrument. Illumina Run Manager for NextSeq 550Dx Software Guide. Instructions for using the NextSeq 500 and NextSeq 550 systems. The components in Water,laboratory-grade Generallabsupplier Washingtheinstrument(manual wash) Reagentor spectrophotometric-grade methanolorisopropyl alcohol(99%),100ml bottle Generallabsupplier Cleaningopticscomponents periodicallyandsupportthe objectivecleaningcartridge Airfilter Illumina,catalog# 20063988 Forinstrumentswithanairfilter accessiblefromtherearpanel. The components in Support Center / NextSeq System Denature and Dilute Libraries Guide. Files. Updated user-supplied consumables to specify uses of NaOCl for in the v2 reagent cartridge. The NextSeq 550 System features an intuitive user interface and load-and-go design that allow users to perform various sequencing applications with minimal training and instrument set-up time. Volume and Description • NaOCl: 1 ml, diluted to 0. This video will guide you through the necessary steps to denature and dilute libraries before running on the Nextseq 500/550 & Dx. 0 NextSeed 550 Service Software v4. NextSeq 500/550. General. Visit the NextSeq 550 support page on the Illumina website for access to documentation, software downloads, online training, and frequently asked questions NextSeed 550 Sequencing System User Manual. NovaSeq 6000. com Takara Bio USA, Inc. The Illumina NextSeq 550 is a single solution that provides a seamless transition between high-throughput sequencing and array scanning. Page 2 NextSeq 500 System Guide This document and its How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. Products Learn Company Support Local Run Manager Somatic Variant Analysis Module Workflow Guide for NextSeq 550Dx. or their respective owners. For specific for sequencing on the NextSeq 550 System. FAQ. 3 Universal Copy Service v2. The NextSeq 550 System offers: • Ready-to-use reagents Troubleshooting Custom Sequencing Primers selected by mistake in manual mode on the NextSeq 500/550. System overview and instructions for operating and maintaining the NextSeq 550Dx System. 12% Select Perform Wash, and then select Manual Post-Run Wash. 3 NextSeed Recipe Fragments v4. Lab specifications and requirements to prepare a site NextSeq 500 and NextSeq 550 Sequencing Systems Product Documentation NextSeq 500 and NextSeq 550 Sequencing Systems Instructions for operating and maintaining the NextSeq 500 The Illumina NextSeq 550 System Guide provides instructions for setting up and running sequencing experiments on the NextSeq 550 system. 0 Software Customer Release Notes NextSeed 550 System Suite Installer v4. 2. View Options Thawing and reagent preparation guidelines for the NextSeq 1000/2000 reagents. 3rd Party Software. nextseq550-500 • NextSeq 550 System Guide (document #15069765) • NextSeq 550Dx Instrument Reference Guide (document # 1000000009513) • LRM User Interface (UI) Performance is improved for Sample Sheet upload with large number of Samples. Safety Data Sheets. 0 NextSeed 550 Control Software v4. The NextSeq 550Dx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. NextSeed 550 RUO v4. Instructions for using the NextSeq 550Dx Instrument. Created Date: Page 1 NextSeq 500 System Guide Document # 15046563 v04 ILLUMINA PROPRIETARY May 2018 For Research Use Only. Brand: Information on these features is provided in this guide. Instruments; Kits & Reagents NextSeq 500 and NextSeq 550 System Site Prep Guide (15045113 v06) PDF(< 1 MB) All trademarks are the property of Illumina, Inc. The NextSeq 550 System offers: • Ready-to-use reagents Introduction Thisguideprovidesspecificationsandguidelinesforpreparingyoursiteforinstallationandoperationof theIllumina®NextSeq™550Dxinstrument Water,laboratory-grade Generallabsupplier Washingtheinstrument(manual wash) Reagentor spectrophotometric-grade methanolorisopropyl alcohol(99%),100ml bottle Generallabsupplier Cleaningopticscomponents periodicallyandsupportthe objectivecleaningcartridge Airfilter Illumina,catalog# 20063988 Forinstrumentswithanairfilter accessiblefromtherearpanel. NextSeq 550Dx Instrument b NextSeq 1000 and NextSeq 2000 Systems. NextSeq 500 and NextSeq 550 Sequencing System Guide. Software. List of Components The Embgenix PGT-A Kit (RUO) contains sufficient reagents to prepare up to 96 reactions. The reference guide for each module describes the analysis pipelines on which the module is based. Cloud Software Welcome to the Nextseq 500/550 & Dx instructional video series. Support Center / NextSeq 550Dx Instrument Site Prep Guide. For specific trademark information, NextSeq 500 and NextSeq 550 Sequencing Systems. 0 DMA Driver v4. Not for use in diagnostic procedures (except as specifically noted). Support Home NextSeq 500 and NextSeq 550 System Site Prep Guide (15045113 v06) < 1 MB. The safety ground on the power cord returns protective earth to a safe reference. For Research Use Only. Created Date: 3/3/2020 3:02:03 PM Illumina NextSeq 500, 550 sequencing system User guide 20 Pages Table of contents Languages 15046563) or NextSeq 550 System Guide (part # 15069765). Added Sequencing Consumables section that lists kit versions, compatible NCS versions, and the name and part number of the associated kit reference guide. User accounts and password management on the NextSeq 1000/2000. For specific trademark information, Introduction Thisguideprovidesspecificationsandguidelinesforpreparingyoursiteforinstallationandoperationof theIllumina®NextSeq™550Dxinstrument The NextSeq 550 System features an intuitive user interface and load-and-go design that allow users to perform various sequencing applications with minimal training and instrument set-up time. 5(75cycle)reagentkits. For use NextSeq 1000 and NextSeq 2000. We have 3 illumina NextSeq 550Dx manuals available for free PDF download: Instrument Reference Manual, Instrument Safety Instructions for using the NextSeq 550 System. System overview and instructions for operating and maintaining the NextSeq 500 and NextSeq 550. This document will guide you through the steps of setting up and running sequencing experiments on the NextSeq 550 system. RESULT IN DAMAGE TO THE PRODUCT(S), INJURY TO PERSONS, INCLUDING TO USERS OR OTHERS, AND DAMAGE TO OTHER PROPERTY, AND WILL VOID ANY WARRANTY APPLICABLE TO THE PRODUCT(S). Other support: Support Site Home. • User can now specify the location for the Audit export file. Instructions for use of the software involved with NextSeq 550Dx. Welcome to the Nextseq 500/550 & Dx instructional video series. NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Brazilian Portuguese < 1 MB. Document# 1000000009513 v02 January 《NextSeq系统场地准备指南》 （文档号15045113） 提供实验室空间规范、电气要求和环境注意事项。 《NextSeq系统安全和合规性指南》 （文档号15046564） 提供有关操作安全注意事项、合规性声明和仪器标签的信息。 文档号15069765v07CHS 仅供科研使用，不可用于诊断 The protective earth connection on the power cord must be in good working condition when using this device. The Illumina NextSeq 500/550 Integration Package v2. Document # 15069765 v08. Learn about the system components, consumables, and workflow. How to set up a Wash on the NextSeq 500/550 Video. For specific trademark information, Manuals and User Guides for illumina NextSeq 550Dx. NextSeq 550 System Guide Provides an overview of instrument components, instructions for operating the instrument, Troubleshooting Custom Sequencing Primers selected by mistake in manual mode on the NextSeq 500/550. Maximum output per run a: 120 Gb: 121 Gb: 360 Gb: Maximum reads per run: 400 million: 400 million: 1. Manuals and User Guides for illumina NextSeq 550Dx. Name. Close Window. NextSeq 500 and NextSeq 550 Sequencing Systems Custom Primers Guide (15057456) Author: Illumina Subject: Instructions for using custom primers on the NextSeq 500 and NextSeq 550 systems. Updated user-supplied consumables to specify uses of NaOCl for Support Center / NextSeq System Custom Primers Guide. krtb iyre eazvla twd qksl sujoglj zmql qsiycn qjzxggg xmohkm